Tottenham chairman Daniel Levy showed London mayor Sadiq Khan around their new stadium on the day it was announced that White Hart Lane will host the first NFL London game of 2018.
Oakland Raiders will take on Seattle Seahawks on October 14 at the 62,000-seater stadium, with the NFL announcing a further two matches to be played at Wembley at the end of October.
Khan said: 'London is the international home of the NFL and I am really excited to welcome the world's best American football stars and their thousands of fans from around the globe back to our city.
'I'm delighted Tottenham Hotspur's fantastic new stadium is joining the line-up of world-class sports arenas in our great city.
'Spurs' new ground will stage one game in its first year of opening and in 2019 we look forward to going back to staging at least four NFL games in London.
Spurs have been playing at Wembley this campaign while their new ground is constructed. They moved out of the old White Hart Lane at the end of last season.
And despite a relatively tight development time for the £750million ground, it looks as though Spurs' new home will be ready for the start of the 2018-19 campaign.
Levy took Khan and NFL executive vice-president Mark Waller around the ground.
Levy said: 'Our vision was always to deliver far more than a stadium – we have looked to create a new sport and leisure destination for London and to start real place change in Tottenham – an area about which we are passionate.
On the NFL fixture, Levy added: 'The announcement of the first game of the NFL's 2018 London Games to take place at our new home heralds the commencement of our 10-year partnership.
'This is one of the cornerstones of our vision for creating a world-class sports and leisure destination that will make Tottenham a hive of activity 365 days a year and drive the regeneration of one of the most deprived areas of London.
By the end of the 2018 season, the NFL will have played 24 regular-season games in London since 2017, with 29 of 32 teams having participated since 2007.
The Wembley matches will see Philadelphia Eagles face Jacksonville Jaguars and Tennessee Titans take on Los Angeles Chargers, with one of the matches played on October 21 and the other on October 28.