Michael Gove has launched a savage attack on 'sneering elites' trying to keep Britain in the EU as he urged voters to 'take back control'.
The Justice Secretary dismissed dire warnings about recession and job losses from organisations such as the Treasury and International Monetary Fund, saying the public was 'sick' of getting told what to do.
He said the EU was a 'job destroying machine' and insisted taxpayers' money should not be going into the pockets of Eurocrats.
But he was branded the 'Oxbridge Trump' by Sky News political editor Faisal Islam, and struggled to defend the Leave campaign's claims that £350million was sent to the EU every week.
The clashes came as Mr Gove appeared on the second Sky News referendum special, following his close friend David Cameron's bruising experience last night.
Mr Gove admitted before taking to the stage that he was 'nervous' about the event, which will see him grilled by members of the public after the interview with Mr Islam.
Both the Remain and Leave camps are hoping that the televised events during the last three weeks of the referendum battle could swing the vote their way.
But Mr Cameron has been heavily criticised for refusing to take part in any head-to-head debates - instead merely agreeing to the Question Time-style programmes.
Mr Cameron was mauled by audience members over his failure to tackle immigration, his Project Fear tactics and 'waffling' last night.
The Prime Minister conceded that inflows from Europe - which amounted to 184,000 last year - were 'challenging'.
He refused to say when he would achieve his long-standing target for slashing net migration overall from the current level of 333,000 to below 100,000. But he insisted that cutting ties with Brussels was not the answer.
Mr Cameron also urged voters to 'look our children, our grandchildren in the eye' before making a decision and not 'roll the dice' with their future.
The appeal came as Mr Cameron - who has long been accused of tightly controlling his contact with ordinary members of the public - faced a series of fiery interventions during the first big televised event of the referendum battle.
Mr Cameron said the EU often drove him 'crazy', and was 'not perfect'.
But he insisted Britons were not 'quitters' and leaving would result in a decade of uncertainty.
He accused Brexit campaigners of repeatedly saying it was 'all going to be okay' rather than spelling out what the country would look like after a Leave vote.
He said 'uncertainty' and 'shock' would hit the economy and prices in the shops would end up being higher.
Mr Cameron clashed with Mr Islam over the PM's claims that leaving the EU would bring 'war and genocide' back to Europe and trigger an economic recession in the UK.
Soraya Bouazzaoui, a second generation Moroccan immigrant from Romford who has just finished an English Literature degree at Solent University, challenged the PM on whether Turkey would be allowed to join the EU.
When Mr Cameron tried to respond by deploying broad arguments for staying in the EU - saying it was important to stay in to 'get things done in the world – Ms Bouazzaoui interrupted by telling the PM: 'You're not answering my question'.
After the programme the student said she had wanted to give Mr Cameron a 'taste of his own medicine' after he spent six years being 'dishonest' with the UK public.
Michael Gove is regarded as one of the deepest thinkers in government.
His decision to join efforts to cut ties with Brussels came despite his close personal friendship with David Cameron.
Mr Gove, another member of Vote Leave's core campaign committee, has mainly been deployed for set-piece speeches, where he has added intellectual weight to the campaign.
However, while widely liked at Westminster Mr Gove is believed to polarise opinion in the country after a controversial spell as Education Secretary.
Mr Cameron shifted him from that high-profile role shortly before the general election for fear he would turn off voters.
Iain Duncan Smith has launched a furious attack on David Cameron for 'deceiving' the public over what powers the government has to curb EU immigration.
In another escalation of Tory tensions over the referendum, the former Cabinet minister accused the Prime Minister of being 'insincere'.
The bitter rowing came after Mr Cameron was taken to task over his failure to cut flows during the first big televised event of the campaign.
The premier conceded that the level of net migration was 'challenging' during the Sky News programme last night - but refused to say when he might be able to hit his target of under 100,000 a year.
Figures last week showed it has been running at 333,000 annually, with 184,000 of those coming from the EU - including 77,000 arrivals who did not have firm jobs lined up.
In an appearance on ITV's Good Morning Britain today, Mr Cameron blamed the strength of the economy for the scale of immigration over recent years.
He said 'wrecking' the economy by leaving the European single market would be a 'terrible way of trying to deal with the issue'.
And in remarks that infuriated Eurosceptics, he claimed that his renegotiation of our membership terms was the best approach to cutting numbers.
‘A better way is what I have negotiated in Europe - is that when people come to work here, if they don't find a job they go home,' Mr Cameron said.
But Vote Leave branded the claims 'lies', insisting that there was no legal mechanism for sending EU nationals home if they did not have a job.
The Brexit campaign also said migrants from the bloc were still entitled to Jobseeker's Allowance after three months in this country, and said Mr Cameron's proposal for curbing benefits for four years would not work.
Mr Duncan Smith said: ‘I’m astonished at these comments, which are deeply insincere - and a clear attempt to deceive the British public.
'The truth is that for as long as we are a member of the European Union we are powerless to control the number of people coming to this country.
'And yesterday’s damning Home Affairs report shows conclusively that even if EU migrants commit serious crimes, the Government is unable to remove them.
‘On his claims about benefits - this is the worst kind of gesture politics.
'I know, because I was in charge of the department responsible for this area.
George Osborne has joined forces with major firms including HSBC, LP Morgan, PwC, Ocado and BT to warn that Brexit could destroy 400,000 jobs in the services sector.
The Chancellor accused the Leave campaign of trying to 'deceive' voters that people's livelihoods are not at risk if we cut ties with Brussels.
The jibe came as Tory infighting over the looming EU referendum raged on.
Former Cabinet minister Iain Duncan Smith has lashed out at David Cameron for being 'insincere' over what powers the UK has to control immigration within the bloc.
The Prime Minister used an appearance on ITV's Good Morning Britain today to argue that his renegotiation of membership terms meant EU nationals could be kicked out if they did not find a job within six months.
The boss of JP Morgan has warned that jobs could be moved to the EU if Britain votes to leave on June 23.
Jamie Dimon said the consequences of a British withdrawal from the EU could have an impact on the investment bank's 16,000 staff in locations in Basingstoke, Bournemouth, Edinburgh, Glasgow, London and Swindon.
Mr Osborne attempted to turn the focus back on to the Remain campaign's favoured turf of the economy during a speech to staff at JP Morgan in Bournemouth.
'Today, 10 of the largest companies in our services sector, from BT to our universities to Universal Music are all telling us that there will be damage to our economy and jobs will be at risk if we leave the EU,' he said.
'So let's end this deception that somehow if we quit the EU that jobs won't be at risk. It's deceiving people to pretend that we can leave the EU and jobs won't be at risk.
'In our analysis of the services sector alone, 400,000 jobs will be at risk.
A letter backing EU membership has been signed by the bosses of HSBC, PwC, Ocado and BT and other leaders of the service sector - which employs more than 25 million people in areas including retail, hospitality, transport, professional and financial services.
'Britain's service sector includes everything from retail to the arts, from finance to education, and from hotels to architects,' they said.
'It accounts for around 80 per cent of value added and 80 per cent of employment, making it the jewel in the crown of British industries.
Jamie Dimon, the CEO of JP Morgan, said the consequences of a British withdrawal from the EU could have an impact on its 16,000 staff in locations in Basingstoke, Bournemouth, Edinburgh, Glasgow, London and Swindon.
He said: 'My observation of the facts is that a vote to leave would be a terrible deal for the British economy.
Saying that the company would have to react quickly to a decision to leave the EU, he added: 'One realistic outcome is that we lose the ability to passport our banking and trading services into Europe.
'But our clients will still need us to trade within what will then be the EU. If that's what the rules say, we will need to do what works.
'So if the UK leaves the EU, we may have no choice but to re-organise our business model here.
But Tory MP Steve Baker said: 'Campaigners for Brussels can't have it both ways.
'They say the EU is about peaceful cooperation, yet then they threaten us if we dare to consider taking back control in favour of a relationship based on trade rather than EU diktats.
'The British people will not be bullied into voting to hand more money and more power to Brussels by someone whose bonus would make even some Eurocrat's eyes water and whose bank helped crash the economy.
Almost 3,500 EU citizens have been sent polling cards for the referendum despite it being illegal for them to vote in the poll, the Electoral Commission admitted tonight.
The elections watchdog yesterday insisted there was only a handful of cases and a memo sent to counting officers, seen by MailOnline, mentioned just seven examples.
But with a number of councils still to confirm the extent of the error, the Electoral Commission tonight issued a statement confirming 3,462 polling cards or postal votes had been issued in error.
EU citizens are not allowed to take part in the referendum because the franchise is based on the system used for the general election franchise.
The Electoral Commission said none of the affected voters would be allowed to take part on polling day.
Yesterday officials reveal a 'glitch' in election software called Xpress used by several councils failed to properly record the nationality of some voters.
The fault was due to have been fixed by a software patch on Wednesday night and any EU voters wrongly issued polling cards or postal votes will be written to with an explanation of what went wrong.
Iain Duncan Smith, the leading Vote Leave campaigner, yesterday wrote to David Cameron to demand a swift investigation into the 'deeply disturbing' claims.
A spokesman for the Electoral Commission said: 'The Commission can today confirm that according to information it has received so far, 3,462 electors were affected by this issue.
'We are still waiting for confirmation from six local authorities about whether they were affected and, if so, the figures will be updated once we receive this information.
'The software provider has resolved the issue which means that, if any postal votes have been issued to these electors, they will be cancelled and none of these electors will be shown as eligible on the electoral registers to be used at polling stations on 23 June.
Donald Trump will fly into Britain the day before the EU referendum after bringing forward his plans to visit his Scottish golf courses.
The Republican presidential candidate had been due to visit Scotland on June 24, the day the result will be announced.
But days after confirming for the first he backed Brexit, Mr Trump revealed his plans to travel had been moved up and become a three day tour of his UK and Ireland businesses.
Senior Remain campaigners warned any intervention by Mr Trump to try and boost Brexit on the eve of polling day would be rejected by 'right minded British citizens'.
David Cameron, who has been embroiled in a war of words with the billionaire over his policy on Muslim immigration, today repeated his willingness to meet candidates for the White House regardless of personal differences between politicians.
But he implied a meeting would have to take place on a later visit - insisting 'campaigning season' was the appropriate time for talks.
Mr Trump tweeted: 'On June 22- I will be going to Scotland to celebrate the opening of the newly renovated Trump Turnberry resort, the worlds best.
'After Trump Turnberry I will be visiting Aberdeen, the oil capital of Europe, to see my great club, Trump Scotland.
'After Trump Scotland, I will visit Trump Doonberg in Ireland, the magnificent resort front on the Atlantic Ocean.
Labour MP Mike Gapes told MailOnline any intervention from Mr Trump would boost the Remain campaign's cause.